Gram staining

  I. Objective: to distinguish between bacteria that are gram positive and gram-negative
 II. Principle: · Gram positive: strong binding Bacteria major paint (g A), not faded with a laxative (g C), and does not bind strong opponents paint (g D) so that the cells of purple bacteria. · Gram negative: Bacteria do not bind the strong primary paint (g A), washed out by a cat laxative (g c), and binds tightly to paint the opponent (g D), so the bacterial cells are red.
     III. Tools and Materials · Sample bacteria · Object glass · Ohse round · Rack painting · Cat: g A, g B, g C, g D · Methylated and methylated burner · Microscope · Oil immersion
      IV. How it Works A. Making Preparations 1. Prepare a glass object clean, dry, fat-free and sterilized over a flame. 2. Write the sample code on the back of the glass object 3. Sterilize ohse above the burner methylated 4. Homogenkan sample, then take 2-3 ohse aseptic bacterial sampling and averaging on the object glass. 5. Wait until dry and then fixation 3x above the burner methylated B. Painting 1. Flood preparations with gram A for 2-5 minutes 2. discard the remaining paint without rinsed, Flood with gram B for 30-40 seconds 3. dispose of the remaining paint, wash with running water, decolorisasi with gram C to fade. 4. wash with running water, Flood with gram D for 2-3 minutes 5. dispose of the remaining paint, wash with running water, dry wind 6. observe under a microscope with 1000x magnification with oil immersion
        V. Results:
Shape: rod Composition: lined Cell color: purple Cat: grams Reaction paint: gram-positive Organelle: - Organelle Color: - Background: pink

      VI. Conclusions: In the samples found bacteria are gram positive rod-shaped
    VII. Discussion: Painting grams Painting was initially developed by experts hitologi namely Christian Gram (1884). Painting gram includes 4 levels: 1. provision of primary paint (paint crystal violet solution, the color purple) 2. intensification of the major paint denganmenambahkan mordan solution 3. laundering (Decolorize) with alcohol solution 4. providing cover paint (paint the opponent) with a solution of safranin red paint. The factors that cause variations in the painting gram: a. Changes in acidity b. Storage ways of painting c. Medium factor d. Age of bacteria e. Special treatment
  VIII. Bibliography
Anonim, 1989, Bakteriologi Umum, Departemen Kesehatan RI : Jakarta